It does not really seem to make a major difference to me, but I do think standing up helps the medicine spread out possibly more and drop to the lower anal area. That is just my opinion. I have been taking these on and off with canasa forever and my cuffitis is still pretty active. I wish I had a better answer for those of us suffering with this chronic, debilitating condition. I have recently started oral pentasa to see if this gives me the little extra boost I need to try and get my cuffitis in remission.

I didn't notice much of a difference depending on body position while on the suppositories. For me it was more about being able to hold the medication in for 4 hours without going to the bathroom. I found 2 hours before bed just after the bathroom trips following dinner was best for me. I would recommend finding the time of the day you frequent the bathroom the least and taking the medicine at the very beginning of that time for the best absorbtion.

I need to lay down when I use Canasa or any suppository, in order to keep it inside long enough for it to do any good. I use it at night when I'm as sure as I can be that my pouch is empty. When I stand up gravity does take over and I need to head to the bathroom.